Background: To test and introduce effective and less toxic breast cancer (BC) treatment\nstrategies, animal models, including murine BC cell lines, are considered as perfect platforms.\nStrikingly, the knowledge on the genetic background of applied BC cell lines is often sparse though\nurgently necessary for their targeted and really justified application. Methods: In this study,\nwe performed the first molecular cytogenetic characterization for three murine BC cell lines C-127I,\nEMT6/P and TA3 Hauschka. Besides fluorescence in situ hybridization-banding, array comparative\ngenomic hybridization was also applied. Thus, overall, an in silico translation for the detected\nimbalances and chromosomal break events in the murine cell lines to the corresponding homologous\nimbalances in humans could be provided. The latter enabled a comparison of the murine cell line with\nhuman BC cytogenomics. Results: All three BC cell lines showed a rearranged karyotype at different\nstages of complexity, which can be interpreted carefully as reflectance of more or less advanced tumor\nstages. Conclusions: Accordingly, the C-127I cell line would represent the late stage BC while the cell\nlines EMT6/P and TA3 Hauschka would be models for the premalignant or early BC stage and an\nearly or benign BC, respectively. With this cytogenomic information provided, these cell lines now\ncan be applied really adequately in future research studies.
Loading....